Ex 5-6. Catalase Test 1. Which of the following types of microbes produces catalase (choose all correct answers) A. obligate aerobes B. facultative anaerobes microaerophiles D. aerotolerants E. obligate anaerobes 2. Which of the following types of microbes produces peroxidase? (choose all correct answers) A. obligate aerobes B. facultative anaerobes microaerophiles D. aerotolerants E. obligate anaerobes 3. You placed one loopful of a bacterial culture into one drop of H2O2, and saw gas bubbles. The gas bubbles must be A. H2 B. CO2 C. O2 D. N2 E. none of these 4. Write the chemical reaction catalyzed by catalase. 5. Write the chemical reaction catalyzed by oxidase. Ex 5-7 Oxidase Test See Figure 5.24 Chemistry of the oxidase reaction 1. The oxidase test is used to detect the presence of oxidase. This enzyme not only reduce cytochrome but also reduce a chromogenic reducing agent and develop color. 2. To perform the test, the oxidase test agent is added to the bacterial culture on a cotton swab or on a growth medium. If the culture turns , it is oxidase positive. This means the bacterium produces cytochrome in its electron transport chain
Added by Jennifer S.
Close
Step 1
Microbes that produce catalase: Catalase is an enzyme that breaks down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). It is produced by organisms that can tolerate oxygen, as hydrogen peroxide is a toxic byproduct of aerobic respiration. Therefore, the Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 96 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
47-Which of the following enzymes are found in aerobic bacteria? catalase B. superoxide dismutase peroxidase D. all of the above 48-When transferring bacteria from one tube to another, it is important to flame the mouth of the tube because a) it helps avoid contamination b) one gets to play with the Bunsen burner c) it helps seal the test tube correctly d) none of the above 49-A basic dye used to stain E. coli is a) eosin b) India ink c) methylene blue d) none of the above 50- E. coli is a gram-negative rod. Part B How do Staph and Strep differ? State ways. How did the MSA medium help us identify S. aureus? From what three sources did we attempt to isolate S. aureus? What is the difference between alpha and beta hemolysis of blood cells?
Adi S.
C) removal of cellular toxins by facultative anaerobes. D) all of above 47. Catalase positive organisms form bubbles when exposed to hydrogen peroxide because? A) They are anaerobic. B) They are aerobic. C) They have enzymes capable of converting H2O2 into O2 and H2O. D) They convert a chromogenic reducing agent. 48. The IMViC series of biochemical tests is used to differentiate and identify: A) enteric bacteria. B) staphylococci. C) streptococci. D) pseudomonads. 49. A dichotomous key: A) is a computer code that is required for access to a microbiology website. B) is a flowchart that tells the microbiologist which tests to perform for identification of an unknown. C) is used to unlock the doors to the Centers for Disease Control and Prevention (CDC) in Atlanta. 50. In the Kirby-Bauer Antibiotic Disk Diffusion Test, susceptibility to an antibiotic is determined by measuring: A) the level of turbidity in a broth culture. B) the size of a zone of growth inhibition on an agar plate. C) a color change in the growth medium.
Sri K.
Farhan A.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD