Exercise 5 Lab Related Question 2 Homework Unanswered Due Feb 18th, 11:59 PM Some of the following steps are used to aseptically transfer a culture to perform the spread plate technique. Order from first to last; note some steps may not be used. Drag and drop options on the right-hand side and submit. For keyboard navigation... SHOW MORE 1 2 3 Add inoculum to a plate with a swab. Use a sterile pipette to add the inoculum to an agar plate. Incubate the plate in an inverted position.
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1. Why are plates inverted (agar side up) while they're incubated? 2. When picking up bacteria growing on a solid medium to transfer to a new culture, do you need to scrape up a huge visible amount? Explain. 3. Instead of incubating a separate control plate, you will leave a space on your plate uninoculated as a control. One reason for this control is to show what color the medium is without microbial growth; why is this necessary? What is a second thing that this control tells you about the experiment?
Madhur L.
Techniques/procedures designed to prevent microbial contamination of sterile equipment, drugs, patients, or pure cultures are called sterile/aseptic techniques.
Sri K.
Put the following steps in order to indicate a correct broth to agar plate transfer. Partially life the lid of the agar plate and with the loop gently streak the surface of the agar plate. Replace the lid. Heat the inoculating loop before replacing it into the rack. Heat the inoculating loop, remove the cap of the culture tube and heat the mouth of the culture tube. Remove a loopful of bacteria from the tube, heat the mouth of the culture tube and replace the cap. If you are using Aseptic Technique in the laboratory, what are you doing? 1) Sterilizing tubes before use in an experiment. 2) Preventing contamination of cultures in the lab. 3) Preventing infectious bacteria from growing in a host organism. 4) Identifying unknown bacterial samples. 5) Incubating samples to encourage bacterial growth. Question 6 (1 point) Bacteria are 'ubiquitous' which means that they are: 1) Found everywhere 2) Able to multiply by mitosis 3) Damaged easily by cleaning chemicals 4) Unable to grow in direct sunlight 5) Resistant to antibiotics
Sahil S.
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