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EXPERIMENTAL SET UP Tube Temperature (°C) ONPG (ml) b-gal (ml) Buffer (ml) Total (ml) A 4 2 2 0 4 B 20 2 2 0 4 C 37 2 2 0 4 D 50 2 2 0 4 E 60 2 2 0 4 F 100 2 2 0 4 G 37 2 0 2 4 H 37 0 2 2 4 I 100 2 0 2 4 RESULTS: Complete this table and submit with your Results section Tube Temperature (°C) Ab420 ONP(µmol)/20 min ONP(µmol)/min A 4 0.12 30 1.5 B 20 0.32 C 37 0.72 D 50 0.20 E 60 0.08 F 100 0.80 G-control 37 0.00 H-control 37 0.00 I-control 100 0.80 ASSIGNMENT PART 2: Report on Student's Own Enzyme Experiment Your report should conform to the standard format of a scientific journal that includes the following sections. You previously submitted a Title and Introduction. In this second part, include Results, Discussion and Literature Cited. Use these terms as headings at the start of each section; they provide an outline for your paper. Suggested length: 2 pages. Your table and graph may be on additional pages. Results: Write this section in the past tense. Before reporting your results, convert absorbance (Ab420) to reaction rate: ONP (µmol)/min. What did you find? Clearly relate your independent variable (temperature) to the reaction rates (dependent variable). Summarize the data in a sentence or two (exact reaction rates NOT needed here). Present the entire data set in a table; include the absorbance as well as the reaction rate in your table (already started for you). Create a graph that includes the experimental treatments (not the controls). Be sure to label the axes with appropriate units: temperature (units?) vs. reaction rate (units?). Save your conclusions (hypothesis) for the Discussion. Discussion: In your first paragraph restate your hypothesis, restate your results and conclude: "These results support (or reject) my hypothesis." If not, why? Explain any unusual results (e.g. 100°C). In another paragraph, tell the reader that the experiment appropriately tested the hypothesis. How? Describe possible problems that might have occurred (at least two) and how you prevented them. Also, point out the various control groups and controlled variables used to prevent any confounding factors (at least four). How could you improve the study, or what is the next step? A final paragraph should make a conclusion that the experiment was appropriate (a summary statement that all factors controlled, etc.) and restate that results supported the hypothesis that ... (restate hypothesis). Literature Cited: Most scientific papers cite several publications (background studies, comparison to other enzymes or organisms, methods used such as your Lab Manual). You should have cited these publications in your Introduction (e.g. Urry et al 2017). Here, provide the complete reference for these sources. Include: author(s), date, title, pages, and either journal or publisher (for books).

          EXPERIMENTAL SET UP

Tube Temperature (°C) ONPG (ml) b-gal (ml) Buffer (ml) Total (ml)
A 4 2 2 0 4
B 20 2 2 0 4
C 37 2 2 0 4
D 50 2 2 0 4
E 60 2 2 0 4
F 100 2 2 0 4
G 37 2 0 2 4
H 37 0 2 2 4
I 100 2 0 2 4

RESULTS: Complete this table and submit with your Results section

Tube Temperature (°C) Ab420 ONP(µmol)/20 min ONP(µmol)/min
A 4 0.12 30 1.5
B 20 0.32
C 37 0.72
D 50 0.20
E 60 0.08
F 100 0.80
G-control 37 0.00
H-control 37 0.00
I-control 100 0.80

ASSIGNMENT PART 2: Report on Student's Own Enzyme Experiment
Your report should conform to the standard format of a scientific journal that includes the following sections. You previously submitted a Title and Introduction. In this second part, include Results, Discussion and Literature Cited. Use these terms as headings at the start of each section; they provide an outline for your paper. Suggested length: 2 pages. Your table and graph may be on additional pages.

Results: Write this section in the past tense. Before reporting your results, convert absorbance (Ab420) to reaction rate: ONP (µmol)/min.
What did you find? Clearly relate your independent variable (temperature) to the reaction rates (dependent variable). Summarize the data in a sentence or two (exact reaction rates NOT needed here). Present the entire data set in a table; include the absorbance as well as the reaction rate in your table (already started for you). Create a graph that includes the experimental treatments (not the controls). Be sure to label the axes with appropriate units: temperature (units?) vs. reaction rate (units?). Save your conclusions (hypothesis) for the Discussion.
Discussion: In your first paragraph restate your hypothesis, restate your results and conclude: "These results support (or reject) my hypothesis." If not, why? Explain any unusual results (e.g. 100°C). In another paragraph, tell the reader that the experiment appropriately tested the hypothesis. How? Describe possible problems that might have occurred (at least two) and how you prevented them. Also, point out the various control groups and controlled variables used to prevent any confounding factors (at least four). How could you improve the study, or what is the next step? A final paragraph should make a conclusion that the experiment was appropriate (a summary statement that all factors controlled, etc.) and restate that results supported the hypothesis that ... (restate hypothesis).
Literature Cited: Most scientific papers cite several publications (background studies, comparison to other enzymes or organisms, methods used such as your Lab Manual). You should have cited these publications in your Introduction (e.g. Urry et al 2017). Here, provide the complete reference for these sources. Include: author(s), date, title, pages, and either journal or publisher (for books).

EXPERIMENTAL SET UP

Tube Temperature (°C) ONPG (ml) b-gal (ml) Buffer (ml) Total (ml)
A 4 2 2 0 4
B 20 2 2 0 4
C 37 2 2 0 4
D 50 2 2 0 4
E 60 2 2 0 4
F 100 2 2 0 4
G 37 2 0 2 4
H 37 0 2 2 4
I 100 2 0 2 4

RESULTS: Complete this table and submit with your Results section

Tube Temperature (°C) Ab420 ONP(µmol)/20 min ONP(µmol)/min
A 4 0.12 30 1.5
B 20 0.32
C 37 0.72
D 50 0.20
E 60 0.08
F 100 0.80
G-control 37 0.00
H-control 37 0.00
I-control 100 0.80

ASSIGNMENT PART 2: Report on Student's Own Enzyme Experiment
Your report should conform to the standard format of a scientific journal that includes the following sections. You previously submitted a Title and Introduction. In this second part, include Results, Discussion and Literature Cited. Use these terms as headings at the start of each section; they provide an outline for your paper. Suggested length: 2 pages. Your table and graph may be on additional pages.

Results: Write this section in the past tense. Before reporting your results, convert absorbance (Ab420) to reaction rate: ONP (µmol)/min.
What did you find? Clearly relate your independent variable (temperature) to the reaction rates (dependent variable). Summarize the data in a sentence or two (exact reaction rates NOT needed here). Present the entire data set in a table; include the absorbance as well as the reaction rate in your table (already started for you). Create a graph that includes the experimental treatments (not the controls). Be sure to label the axes with appropriate units: temperature (units?) vs. reaction rate (units?). Save your conclusions (hypothesis) for the Discussion.
Discussion: In your first paragraph restate your hypothesis, restate your results and conclude: "These results support (or reject) my hypothesis." If not, why? Explain any unusual results (e.g. 100°C). In another paragraph, tell the reader that the experiment appropriately tested the hypothesis. How? Describe possible problems that might have occurred (at least two) and how you prevented them. Also, point out the various control groups and controlled variables used to prevent any confounding factors (at least four). How could you improve the study, or what is the next step? A final paragraph should make a conclusion that the experiment was appropriate (a summary statement that all factors controlled, etc.) and restate that results supported the hypothesis that ... (restate hypothesis).
Literature Cited: Most scientific papers cite several publications (background studies, comparison to other enzymes or organisms, methods used such as your Lab Manual). You should have cited these publications in your Introduction (e.g. Urry et al 2017). Here, provide the complete reference for these sources. Include: author(s), date, title, pages, and either journal or publisher (for books).

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