5. Explain how DNA is transcribed into RNA by referring to: i. The signals on the DNA that are recognized during the transcription process; ii. The enzymes that are involved during this process; iii. The final structure of the mature mRNA. 6. Name the three steps of the transcription process and discuss each step briefly. 7. Discuss the most important differences between the transcription process in eukaryotes and prokaryotes. 8. Explain the various post-transcriptional modifications which the primary transcription product in eukaryotes undergo to form the final mRNA. 9. Describe in detail the synthesis of an aminoacyl-tRNA and the specific role of loaded tRNAs in the translation process. 10. Name the components of a ribosome. 11. Explain how the translation-initiation complex is formed. 12. Describe the process of chain elongation and chain termination during the translation process. 13. Rewrite any given DNA sequence into its RNA complement according to the universally accepted rule. If the rewritten piece of RNA represents the 5' end of an mRNA molecule, with both a 5' non-encoding area and part of a gene encoding area, you need to be able to use the codon table for mRNA to determine what the amino acid sequence of the corresponding polypeptide would be that would be translated from this mRNA template. 14. Explain which types of mutation within the coding area of a gene can generally influence the final protein product. 15. Determine the corresponding change in the amino acid sequence of the peptide if any of several different mutations should occur in a given nucleotide sequence.
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The enzymes that are involved during this process are the RNA polymerase and the ribosomes. The final structure of the mature mRNA is composed of a Show more…
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Transcription and Translation 1. In transcription...what is copied? What is made? 2. What is the role of the "promoter", "terminator" and mRNA in transcription? 3. Explain why the genetic code is a "triplet" code using math! :-) 4. Below is a DNA sequence. Envision that this is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the antisense strand. Construct the mRNA sequence transcribed from this template: Antisense DNA strand: 3'-T A C T G A C T G A C G A T C-5' 5. Define: codon, anticodon, and nonsense codon. 6. What is the advantage of having a "degenerate" genetic code? 7. Describe the process of "initiation", "elongation" and "termination" in translation 8. Below is an antisense DNA sequence. Translate the mRNA molecule synthesized using the genetic code, recording the resulting amino acid sequence, indicating the N and C termini. You will need the genetic code found HERE in your textbook: Antisense DNA strand: 3'-T A C T G A C T G A C G A T C-5' 9. Why can translation begin before transcription is complete in prokaryotes, but not eukaryotes?
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Help please also can you explain if you can Instructions: For this assignment, you will replicate DNA to make a complimentary strand of DNA. Then transcribe the complimentary DNA sequence into mRNA. Next, translate the mRNA molecule into the codes for specific amino acids using the amino acid chart on the last page. Lastly, address the reflection questions. DNA Replication: Replicate the following strand of DNA: Original DNA A T G A A C C A T T C A G T A T G G Complimentary DNA Transcription: Transcribe the DNA to make an mRNA molecule Complimentary DNA mRNA Molecule Translation: Translate the mRNA into the corresponding amino acids. mRNA Molecule Amino Acid Using the DNA sequence provided, determine the amino acids. Original DNA A T G G G T C T A G C G A A A G A T Complimentary DNA mRNA Molecule Amino Acid Questions: Using what you have learned in the lesson and the experiment, answer the following questions in complete sentences. What is the function of mRNA? What is the function on tRNA? Describe what happens during transcription. Describe what happens during translation. Explain how DNA is used to make a protein.
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