00:01
So the key ingredients of pcr.
00:05
The ingredients of pcr reaction, polymerase chain reaction are tack polymerase, primers and template.
00:26
So the tap polymerase enzyme primers and the template dna and the nucleotides, dna building blocks.
00:44
Other than the template nucleotides, dna building box are important or the key ingredients of polymer is chain reaction.
00:55
And these ingredients are assembled in a tube like structure.
01:02
These are called small pcr tubes.
01:05
These are smaller than the microfuge tubes and all these ingredients are one by one put into the tubes.
01:14
Along with the cofactors that are needed by the enzyme are put through the repeated cycles of heating and cooling that allow the dna to be synthesized.
01:25
So the basic steps are the first step is denaturation.
01:39
So this happens at, this denaturation happens at 96 degrees celsius and in this reaction that the heat the the heat reaction strongly tends to separate the strands of dna or it basically denatures the dna strands the dna strands are like this they are born together by h bonds and after the denaturation process at 96 degrees the dna strands become denatured.
02:25
That is the hydrogen bonds are broken due to heat.
02:33
So this is what will happen after the denaturation step.
02:37
This provides a single stranded dna...