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EXTRA PRACTICE: DNA REPLICATION REVIEW MATCHING For each of the molecules listed, choose the description that best describes its function in DNA replication. Molecule: 1. DNA ligase 2. Helicase 3. DNA Polymerase I 4. DNA Polymerase III 5. Single-stranded binding proteins 6. Primase Function: a. unwinds the double helix b. joins gaps in the sugar-phosphate backbone c. attaches the RNA primer d. replaces RNA primer with DNA nucleotides e. constructs a new DNA strand in the 5' to 3' direction f. keeps the double helix unwound SHORT ANSWER One half of a replication bubble (note origin of replication) is illustrated below. 7. Label the leading and lagging strands in this bubble. 8. Label the 5' and 3' ends of both the template (parent) and new strands. 9. Using the following list of terms, identify each of the proteins/enzymes labeled in the diagram below. helicase single-stranded binding proteins DNA polymerase I DNA polymerase III DNA ligase primase 10. For those terms you did not use in #9, indicate of the diagram above exactly where they would work (and what they would do). 11. On the replication bubble below, where would you expect to see Okazaki fragments? a. closest to (a) and (b) b. closest to (c) and (d) c. closest to (e) and (f) d. closest to (a) and (d) e. closest to (b) and (c) NOTE: DNA POLYMERASE I = DNA POLYMERASE W/EXONUCLEASE Origin of replication

          EXTRA PRACTICE: DNA REPLICATION REVIEW
MATCHING
For each of the molecules listed, choose the description that best describes its function in DNA replication.
Molecule:
1. DNA ligase
2. Helicase
3. DNA Polymerase I
4. DNA Polymerase III
5. Single-stranded binding proteins
6. Primase
Function:
a. unwinds the double helix
b. joins gaps in the sugar-phosphate backbone
c. attaches the RNA primer
d. replaces RNA primer with DNA nucleotides
e. constructs a new DNA strand in the 5' to 3' direction
f. keeps the double helix unwound
SHORT ANSWER
One half of a replication bubble (note origin of replication) is illustrated below.
7. Label the leading and lagging strands in this bubble.
8. Label the 5' and 3' ends of both the template (parent) and new strands.
9. Using the following list of terms, identify each of the proteins/enzymes labeled in the diagram below.
helicase
single-stranded binding proteins
DNA polymerase I
DNA polymerase III
DNA ligase
primase
10. For those terms you did not use in #9, indicate of the diagram above exactly where they would work (and what they would do).
11. On the replication bubble below, where would you expect to see Okazaki fragments?
a. closest to (a) and (b)
b. closest to (c) and (d)
c. closest to (e) and (f)
d. closest to (a) and (d)
e. closest to (b) and (c)
NOTE: DNA POLYMERASE I = DNA POLYMERASE W/EXONUCLEASE
Origin of replication

EXTRA PRACTICE: DNA REPLICATION REVIEW
MATCHING
For each of the molecules listed, choose the description that best describes its function in DNA replication.
Molecule:
1. DNA ligase
2. Helicase
3. DNA Polymerase I
4. DNA Polymerase III
5. Single-stranded binding proteins
6. Primase
Function:
a. unwinds the double helix
b. joins gaps in the sugar-phosphate backbone
c. attaches the RNA primer
d. replaces RNA primer with DNA nucleotides
e. constructs a new DNA strand in the 5' to 3' direction
f. keeps the double helix unwound
SHORT ANSWER
One half of a replication bubble (note origin of replication) is illustrated below.
7. Label the leading and lagging strands in this bubble.
8. Label the 5' and 3' ends of both the template (parent) and new strands.
9. Using the following list of terms, identify each of the proteins/enzymes labeled in the diagram below.
helicase
single-stranded binding proteins
DNA polymerase I
DNA polymerase III
DNA ligase
primase
10. For those terms you did not use in #9, indicate of the diagram above exactly where they would work (and what they would do).
11. On the replication bubble below, where would you expect to see Okazaki fragments?
a. closest to (a) and (b)
b. closest to (c) and (d)
c. closest to (e) and (f)
d. closest to (a) and (d)
e. closest to (b) and (c)
NOTE: DNA POLYMERASE I = DNA POLYMERASE W/EXONUCLEASE
Origin of replication

Added by Brooke T.

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