Fermenter KLa Oxygen is one of the most difficult nutrients to provide in a fermenter. This is because oxygen is sparingly soluble in water. Oxygen must diffuse from a bubble, into the media and then finally to the cell. The KLa of a reactor, is the measure of how well a reactor, at a giving setting, can transfer oxygen. An experimental design was set up to investigate three factors that might be important. Using the data in Figure I, determine the main factors and two factor interactions that are important. Experimental Design Fermentor KLa Run # RPM Vol. Flow(L/min) Pressure(gauge) KLa (hr-1) 1 400 4 0 psi 39.6 2 400 4 5 psi 39.6 3 400 8 0 psi 32.4 4 400 8 5 psi 36 5 550 4 0 psi 57.6 6 550 4 5 psi 57.6 7 550 8 0 psi 75.6 8 550 8 5psi 79.2
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First, we need to understand what the three factors are in this experiment. From the data provided, it seems the three factors are: RPM (Revolutions Per Minute), Volume Flow (L/min), and Pressure (gauge). Show more…
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