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Figure 1. Questions 1-4. DNA from wheat germ was digested with micrococcal nuclease and the resulting fragments were separated by agarose electrophoresis on a 1.5% gel. Lanes 2, 3, 4 and 5 contain samples of DNA that were digested with 20 uL of nuclease. The length of the incubation time of the enzyme with the samples varied. Lane 1 contains a 100 bp ladder. Lane: 1 2 3 4 5 600 bp 400 bp 200 bp 100 bp 1. Please explain the dark smear in the upper portion of lane three. [Insert answer here] 2. Lane 3 appears to have a dark band in the well at the top of the gel, how do you explain this? [Insert answer here] 3. On the gel, label a band that contains DNA fragments that were wrapped around 2 nucleosomes plus the linker DNA [units should be in base pairs]. Approximately how many base pairs do you assume this to be, why? [Insert answer here] 4. This gel seems to have some poor resolution of gel bands in both lanes 2 and 3. Assuming that you have to run the samples together in a new gel, what could you do to optimize the resolution of the DNA fragments in lane 2 and lane 3? [Insert answer here]

          Figure 1. Questions 1-4. DNA from wheat germ was digested with micrococcal nuclease and the resulting fragments were separated by agarose electrophoresis on a 1.5% gel. Lanes 2, 3, 4 and 5 contain samples of DNA that were digested with 20 uL of nuclease. The length of the incubation time of the enzyme with the samples varied. Lane 1 contains a 100 bp ladder.

Lane: 1 2 3 4 5

600 bp
400 bp
200 bp
100 bp

1. Please explain the dark smear in the upper portion of lane three.

[Insert answer here]

2. Lane 3 appears to have a dark band in the well at the top of the gel, how do you explain this?

[Insert answer here]

3. On the gel, label a band that contains DNA fragments that were wrapped around 2 nucleosomes plus the linker DNA [units should be in base pairs]. Approximately how many base pairs do you assume this to be, why?

[Insert answer here]

4. This gel seems to have some poor resolution of gel bands in both lanes 2 and 3. Assuming that you have to run the samples together in a new gel, what could you do to optimize the resolution of the DNA fragments in lane 2 and lane 3?

[Insert answer here]

Figure 1. Questions 1-4. DNA from wheat germ was digested with micrococcal nuclease and the resulting fragments were separated by agarose electrophoresis on a 1.5% gel. Lanes 2, 3, 4 and 5 contain samples of DNA that were digested with 20 uL of nuclease. The length of the incubation time of the enzyme with the samples varied. Lane 1 contains a 100 bp ladder.

Lane: 1 2 3 4 5

600 bp
400 bp
200 bp
100 bp

1. Please explain the dark smear in the upper portion of lane three.

[Insert answer here]

2. Lane 3 appears to have a dark band in the well at the top of the gel, how do you explain this?

[Insert answer here]

3. On the gel, label a band that contains DNA fragments that were wrapped around 2 nucleosomes plus the linker DNA [units should be in base pairs]. Approximately how many base pairs do you assume this to be, why?

[Insert answer here]

4. This gel seems to have some poor resolution of gel bands in both lanes 2 and 3. Assuming that you have to run the samples together in a new gel, what could you do to optimize the resolution of the DNA fragments in lane 2 and lane 3?

[Insert answer here]

Added by Timothy H.

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