16. Kligler's Iron agar, because of its composition, can be used for which of the following applications? A. Testing for Hydrogen Sulfide production B. Testing for Glucose fermentation C. Testing for Lactose fermentation D. Can be used for all of the above 17. A positive reaction for Citrate utilization will produce a color change A. From deep blue to green B. From red to yellow C. From colorless to red D. From green to deep blue Answer True (A) or False (B) to the following questions 18. _____ A bacterial culture grown in Nitrate broth gives a red coloration on addition of Nitrate reagents A and B. This indicates positive reaction for Nitrate oxidation 19. _____ Pseudomonas aeruginosa is positive for Oxidase activity 20. _____ Streptococcus agalactiae is classified Group B beta hemolytic 21. _____ Streptococcus pyogenes is classified as Group A alpha hemolytic 22. _____ Staphylococcus aureus is both mannitol fermenting and coagulase positive 23. Which of the following is a selective and differential medium for the genus Staphylococci? A. Mannitol Salt Agar B. MacConkey Agar C. M Staphylococcal Medium D. Both A and C E. All of the above 24. Mannitol Salt Agar contains mannitol, 7.5% sodium chloride, and phenol red. The role of sodium chloride in the medium is A. To select for all gram positive bacteria B. To select for all gram negative bacteria C. To inhibit the growth of organisms other than Staphylococci D. To differentiate Mannitol Salt agar (MSA) from Staphylococcus medium 110 (SM 110) 25. Which of the following is not a selective and differential medium for the growth of Gram negative bacteria? A. MacConkey Agar B. Hektoen Agar C. Xylose Lysine Desoxycholate (XLD) Agar D. Fluid Thioglycollate Medium (FTM)
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Instructions: Choose 1 appropriate biochemical test for following samples. Sample 1: from a contaminated small body of water in Mt. Makiling Sample 2: sludge from a restaurant Biochemical Test: 1. Molisch Test (Test for carbohydrates) 2. Biuret Test (Test for proteins) 3. Liebermman-Burchard Test (Test for lipids) 4. Dische Reaction (Test for DNA) 5. Bialās Orcinol Test (Test for RNA) 6. Molisch (General test for sugars, Formation of furfural derivatives) 7. Bialās Orcinol Test (Test for pentoses) 8. Seliwanoff's Test (Distinguish bet aldose and ketose sugar) 9. Benedict's Test (Test for reducing sugars, Reduction of alkaline copper solutions by sugars with free aldehyde or ketone group) 10. Barfoed's (Differentiation of mono from disaccharides, Copper ions detect reducing sugars in an acidic solution) 11. Tollen's (Test for reducing sugars, Reduction of Ag+ to Ag) 12. Xanthoproteic Test (Detects benzene ring) 13. Millonās Test ( Detects phenolic āOH Group) 14. Hopkinās Cole Test (Detects indole ring) 15. Lead Acetate (a.k.a. Sulfur) Test ( Detects sulfur-containing amino acids) 16. Ninhydrin Test (Detects amine group) 17. Biuret (Detects at least two peptide bonds) 18. Denaturation ( Destruction of peptide bonds, breaking down proteins into amino acids (negative Biuret test)) 19. Precipitation w/ Heavy Metal Salts (Free carboxylate anions of acidic amino acids precipitate heavy metals(cations)) 20. Qualitative Tests for Nucleic Acids (Test for any of the nucleic acid/nucleotide components: Nucleoproteins (histones), Mild acid hydrolysis to separate: Inorganic phosphates, Ribose (pentose), Purines) 21. Biuret Test for Nucleoproteins (NaOH and CuSO4) 22. Test for Inorganic Phosphates (Ammonia, HNO3 and ammonium molybdate) 23. Bialās Orcinol Test for Ribose (Pentose) (Orcinol + HCl) 24. Test for Purines (NH4OH and AgNO3)
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