For example, if you count 50 colonies on a plate where you plated 100 µL (aka 0.1) from a 10-6 dilution, the calculation would be:
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If you count 60 colonies after generating a dilution factor of 10^-8 and plating 100 microliters, what is the starting concentration?
Sri K.
Dilution plating. You count 52 colonies on the 1/10,000 dilution plate. Original number of CFU/ml would be... 52 CFU/ml 520 CFU/ml 5,200 CFU/ml 52,000 CFU/ml 520,000 CFU/ml 5,200,000 CFU/ml
Adi S.
Now Calculate the CFU/ml CFU/ml= X colonies x 1/DF First calculate the average colonies from the two plates. Next calculate the DF DF = Dilution Factor DF = amount plated x the dilution plated. Last step is to plug in our numbers.
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