1. You work in the morgue in Havana as a forensic pathologist. Given Arjun's risk factors, possible exposures, and symptoms, what are the five most likely infectious agents (names, specific species) that killed him? Please explain your reasoning (multiple right answers if you can support why it's likely). (15 points)
2. You analyzed the blood work that the ship's doctor had performed. This helped to narrow down possible causes of Arjun's death. How did you know this? (10 points)
* Platelets - 29 x 10^9/L
* C3b/C4b - 2.4 g/L and 2.1 g/L respectively
* Atypical lymphocytes - 21%
* Neutrophils - 10.0 x 10^9/L
* Lymphocytes - 5.8 x 10^9/L
3. You took samples from his lesions and lungs and conducted RT qPCR. The results are below. As a confirmation, you used an ELISA to show the tissue was positive for antibodies to gE. Describe these assays and interpret these results. You now know what killed him! (15 points, use the Chapter 28 video lecture to help here)
4. What are the basic properties of this pathogen? (5 pt)
5. What was this pathogen doing in Arjun's body to cause disease? (pathogenesis, virulence factor names, use Chapter 25 and google scholar) (20 points)
6. How did Arjun's innate immune system try to protect him from the infection? (make sure it is both general version from the 26/7 lecture and also via research, specific for this type of attack) (10 points)
7. Please describe the acquired immune reaction that occurred in response to this infection. (make sure it from the 26/7 lecture, as if he had been vaccinated, and also via research, specific for this type of attack, i.e. how it won and killed him) (20 points)
8. If he had been your patient from the first onset of symptoms, what would you have done to prevent his death? How does this treatment work at a molecular level? (5 pt, use internet resources)