Question

GENETICS, Thank You! For the lac operon in E. coli, I = lacI, the repressor gene, P = the lac promoter, O = the lac operator, Z = lacZ or the beta-galactosidase gene, Y = lacY, the permease gene. (+ = gene intact or functional; - = gene deleted or nonfunctional). Fill in the table below using a '+' to indicate the presence of the enzyme and '-' to indicate the absence of the enzyme. Although there is residual expression in the absence of lactose, please call this '-' since there is a thousand-fold increase in the presence of lactose. Genotype No Lactose Lactose Lac Z Lac Y Lac Z Lac Y I+P-O+Z+Y+ I+P+O+CZ+Y+ I+P+O+Z+Y- /F' I-P+O+Z-Y+ I-P+OcZ+Y+ /F' IsP+O+Z-Y- I+P+O+Z-Y+ /F' I-P+O+Z+Y-

          GENETICS, Thank You! For the lac operon in E. coli, I = lacI, the repressor gene, P = the lac promoter, O = the lac operator, Z = lacZ or the beta-galactosidase gene, Y = lacY, the permease gene. (+ = gene intact or functional; - = gene deleted or nonfunctional). Fill in the table below using a '+' to indicate the presence of the enzyme and '-' to indicate the absence of the enzyme. Although there is residual expression in the absence of lactose, please call this '-' since there is a thousand-fold increase in the presence of lactose.

Genotype
No Lactose
Lactose
Lac Z
Lac Y
Lac Z
Lac Y
I+P-O+Z+Y+
I+P+O+CZ+Y+
I+P+O+Z+Y-
/F'
I-P+O+Z-Y+
I-P+OcZ+Y+
/F'
IsP+O+Z-Y-
I+P+O+Z-Y+
/F'
I-P+O+Z+Y-

Added by Suzanne H.

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