Given the amino acid sequence of a polypeptide: Phe, Val, Asn, Gln, His, Leu, Cys, Gly, Ser
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First, we need to create a list of the given amino acid sequence. Show more…
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Determine the amino acid sequence of a polypeptide from the following data: Complete hydrolysis of the peptide yields Arg. 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val. Treatment with cyanogen bromide yields the following three peptides: 1. Gly-Leu-Tyr-Phe-Lys-Ser-Met 2. Gly-Leu-Tyr-Lys-Val-Ile-Arg-Met 3. Leu-Pro-Phe Treatment with trypsin yields the following four peptides: 1. Gly-Leu-Tyr-Phe-Lys 2. Ser-Met-Gly-Leu-Tyr-Lys 3. Val-Ile-Arg 4. Met-Leu-Pro-Phe
Determining the amino acid sequence in a protein usually involves treating the protein with various reagents that break up the protein into smaller fragments, which can be individually sequenced. Treating a particular 11 -amino-acid polypeptide with one reagent produced these fragments: trp-glu-val, gly-arg, ala-ser-phe-gly-asn-lys Treating the same polypeptide with a different reagent produced the following fragments: gly-asn-lys-trp, glu-val, gly-arg-ala-ser-phe What is the amino acid sequence of the polypeptide?
3. Use the coding sequence below to answer the following questions. mRNA codons: UUA-CCU-GUU-AUU Amino acids: leu-pro-val-thr a. A guanine nucleotide was inserted at the beginning of this sequence as shown. Rewrite the mRNA codons with this insertion. Then, write the corresponding amino acids that would result from this addition. mRNA codons (with insertion): Amino acids: b. What type of mutation would this insertion produce?
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