Given the result of bisulfite treatment and sequencing, indicate the nucleotides that were methylated in the heart cells.
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This allows for the differentiation between methylated and unmethylated cytosines after sequencing. Show more…
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Describe the analysis of nucleotide methylation with the bisulfite method. What nucleotides Would you expect to be modified?
Adi S.
A geneticist is interested in determining the locations of methylated cytosines within a fragment of DNA. She treats some copies of the fragment with sodium bisulfite and leaves some copies untreated. She then sequences the treated and untreated copies of the fragment and obtains the following results. Give the original sequence of the DNA frag ment and indicate the presence of methylated cytosines. Sequence without treatment: -AATTGCCCGATCGATTAAGCCA- Sequence with treatment: -AATTGTTTGATCGATTAAGCTA-
A DNA fragment with the following base sequence has some cytosine bases that are methylated (indicated by $\left.\mathrm{C}^{*}\right)$ and others that are unmethylated. To determine the location of methylated and unmethylated cytosines, researchers sequenced this fragment both with and without treat ment with sodium bisulfite. Give the sequence of bases that will be read with and without bisulfite treatment. -ATCGC'GITAC'ATTGC'ATCA-
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