gram staining procedure1 what is the theory about the mechanism of the gram stain reaction2 whic

Step 1: The theory behind the mechanism of the Gram-stain reaction is based on the differences i

Question

Gram Staining Procedure 1. What is the theory behind the mechanism of the Gram-stain reaction? 2. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 3. Why must young cultures be used when doing a Gram stain? 4. What part of the bacterial cell is most involved in Gram staining, and why? 5. What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected? 6. What is the purpose of the alcohol solution in the Gram stain? 7. What counterstain is used? Why is it necessary? Could colors other than red be used? 8. What would be the function of heat-fixing in the preparation of a smear? 9. What do you think would be easier to prepare a bacterial smear from, liquid or solid culture? 10. Aside from bacteria, do you think that Gram staining can be used with microbes such as fungi? Explain briefly your answer.

          Gram Staining Procedure
1. What is the theory behind the mechanism of the Gram-stain reaction?
2. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why?
3. Why must young cultures be used when doing a Gram stain?
4. What part of the bacterial cell is most involved in Gram staining, and why?
5. What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected?
6. What is the purpose of the alcohol solution in the Gram stain?
7. What counterstain is used? Why is it necessary? Could colors other than red be used?
8. What would be the function of heat-fixing in the preparation of a smear?
9. What do you think would be easier to prepare a bacterial smear from, liquid or solid culture?
10. Aside from bacteria, do you think that Gram staining can be used with microbes such as fungi? Explain briefly your answer.

Added by Heather R.

Question

Please give Ace some feedback