Heat-denatured DNA exhibits ______ UV absorbance, which is called the ______ increased; hyperchromic effect decreased; hyperchromic effect increased; hypochromic effect decreased; hypochromic effect identical; renaturation effect
Added by Tammy W.
Close
Step 1
Step 1: When DNA is heated, the hydrogen bonds between the base pairs break, causing the double helix to unwind and separate into single strands. Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 82 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
What is the hyperchromic effect? A hyperchromic effect is the increased absorption of UV light as double-stranded DNA is converted to single-stranded DNA. A hyperchromic effect is the decreased absorption of UV light as single-stranded DNA is converted to double-stranded DNA. A hyperchromic effect is the decreased absorption of UV light as double-stranded DNA is converted to single-stranded DNA. A hyperchromic effect is the increased absorption of UV light as single-stranded DNA is converted to double-stranded DNA. What does Tm imply? The Tm is the temperature at which half of the sample is denatured. The Tm is the temperature at which the sample starts to denature. The Tm is the maximum temperature at which the sample does not denature. The Tm is the temperature at which all the sample is denatured.
Adi S.
Which statements about the phenomena illustrated in the following graph is/are TRUE? Single-stranded DNA Double-stranded DNA Protein 230 240 250 260 270 280 290 Wavelength (nm) Select all that apply: Proteins absorb u.v. light with a peak at 260 nm. ds DNA is hypochromic compared with single stranded nucleic acids. ds RNA behaves like ds DNA upon melting Protein contaminants in a DNA sample would increase the apparent A260 nm. RNA contaminants in a DNA sample would reduce the apparent A260 nm.
A hyperchromic shift in UV-Vis results in: a red shift in the lambda max no change a blue shift in the lambda max lower molar absorptivity constant A higher molar absorptivity constant
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD