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1. First, we need to correct for the dilution error. The procedure called for a 1/5 dilution, bu

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1. You are performing a urine analysis. The procedure calls for a 1/5 dilution but you made a 1/10. Then the procedure requires 0.5 mL but you used 0.2 mL. The glucose level is 60 mg/dL. What should be reported out? (Report in a whole number) 2. 7.5% saline was diluted 1/10 then 1/50. What is the concentration of the final solution? (round off to the hundredths) 3. You are to make a serial dilution using 10 tubes. Tube 1 has 9 mL of broth. All the others have 1 mL. One mL of bacterial culture is added to TUbe 1. One mL from this tube is transferred to the 2nd tube and so on. What is the solution dilution for tube 1, 4, & 8? 4. A protein free filtrate of a specimen was made by adding 0.1 mL specimen, 3.4 mL sodium tungstate and 4.5 mL H2SO4. What factor will you multiply the result by to correct for the procedure?

Transcript

00:01 Number one, you're performing a urine analysis.

00:03 The procedure calls for 1 5th dilution, 1 of 5 dilution, but you made a 1 of 10.

00:08 Then the procedure requires 0 .5 ml, but you only use 0 .2.

00:11 The glucose level is 60 milligrams per deciliter.

00:14 What should be reported out? so first of all, you're going to have to use the concentration, 60 milligrams per deciliter divided by the dilution, which is 10.

00:37 So you get 6 milligrams per deciliter.

00:40 However, you use less volume.

00:44 So the adjusted factor is 0 .5 ml divided by 0 .2 ml because you use less volume.

00:52 So this is actually 2 .5.

00:55 So this is the factor.

00:57 So obviously, you have to take the adjusted factor into consideration, 6 milligrams per deciliter times 2 .5.

01:07 You get 15 milligrams per deciliter.

01:12 So this is what you should report out.

01:16 Second, 7 .5 % saline was diluted 1 of 10, then 1 to 50.

01:22 What is the concentration of the final solution? so you have a 1 of 10 dilution first, and then you have 1 of 50.

01:35 So the final dilution is 1 of 10 times 1 of 50.

01:42 So it's 1 to 500.

01:45 So this is the final dilution factor.

01:58 Now, if you start out with 7 .5%, the final concentration is going to be diluted.

02:10 Concentration is going to be the initial concentration divided by the dilution factor.

02:20 So we know the initial concentration is 7 .5%, and the dilution factor is 500.

02:37 So you get 0 .015%.

02:50 And if you want to round off to the 100th, then you have to have only two decimals, 0 .02%.

03:01 So you run it up.

03:03 The next question, you are making a 0 dilution using 10 tubes.

03:13 Tube 1 has 9 milliliter of broth, and all the others have 1.

03:20 So 1 milliliter of bacteria is added to tube number 1.

03:23 So that means 1 goes into 1 plus 9 total volume.

03:30 So this is a 1 to 10 dilution.

03:32 This is a tube 1.

03:37 Now, 1 milliliter from this tube is transferred to the second tube and so on.

03:43 So basically, the rest of them, you have 1 milliliter of culture instead of 9.

03:51 So then the next one, tube number 2, is 1 into 1 plus 1 milliliter total volume.

04:01 So it's a 1 to 2 dilution compared to tube number 1.

04:06 So this is a tube 2.

04:10 And so go on.

04:12 The next one, you take 1 out and add it into another 1.

04:17 So it's 1, i guess i would just say 1 plus 1 again...

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