Histidine is one of twenty natural amino acids and possesses three acidic protons (pKa1 = 1.82, pKa2 = 6.00, pKa3 = 9.17).
a) Starting with 10.0 mL of 0.325 M histidine, determine the pH (two decimal places) of this solution (Hint: the first pKa is the main contributor of hydronium concentration).
b) Using 0.100 M NaOH as a titrant, at what volumes are the 1st, 2nd, and 3rd equivalence points reached (two decimal places)?
1st Veq =
2nd Veq =
3rd Veq =
c) What are the pH values at the 1st, 2nd, and 3rd mid-equivalence points (two decimal points)?
1st pH1/2eq =
2nd pH1/2eq =
3rd pH1/2eq =
d) What are the pH values at the 1st and 2nd equivalence points?
1st pHeq =
2nd pHeq =
e) What is the pH at the third equivalence point?
3rd pHeq =