How are many cultures of microbes routinely preserved? Multiple choice question. Dessication Solid agar plates Freezing Liquid culture
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Microbes can be preserved for research, industrial, or clinical purposes to maintain their viability and characteristics over time. Show more…
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What is a common method of preserving bacterial cultures for very long periods of time? Multiple Choice desiccation refrigeration saponification lyophilization
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please select two methods that can be used for long term preservation of microbial cultures
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1. Agar media can be enriched with blood for the identification of: - Viruses from other microorganisms - Bacteria which can grow in anaerobic conditions - Bacteria which can grow in higher hydrostatic pressure - Bacteria which can cause hemolysis 2. Isolation of pure culture refers to: - Growing microorganisms on a surface - Separation of a single colony - Introduction of inoculum - Purification of culture 3. A rapid qualitative isolation method involving spreading a loopful of inoculum over the surface of an agar plate is known as: - Stabbing - Identification - Streaking - Isolation 4. A subculture is a: - Culture made from a contaminant - Culture made in an embryo - Culture made from an isolated colony - Colony growing beneath the media surface 5. Which of the following methods can be used to determine the number of bacteria quantitatively? - Streak-plate - Pour plate - Pour-plate and spread plate - Spread-plate 6. Addition of blood to culture medium only allows the hemolytic bacteria that grow on a plate to be picked out. This is an example of: - Selective media - Differential media - Chemically defined media - Complex media 7. Elective media facilitate the growth of only one kind of organism. Sabouraud medium is used to selectively isolate: - Acid-fast organisms - Gram-positive bacteria - Coliform bacteria - Yeasts 8. In the pour-plate method, the medium should be maintained at what temperature? - 45 degrees C - 0 degrees C - 37 degrees C - 67 degrees C 9. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium known as: - Contamination - Disinfection - Inoculation - Isolation 10. Which of the following refers to specific procedures used to prevent unwanted microorganisms from contaminating the clinical specimen? - Inoculation - Disinfection technique - Aseptic technique - Needle technique 11. Bacteria that require special growth factors and complex organic substances are called: - Pathogenic - Aerobic - Chemoheterotroph - Anaerobic - Fastidious 12. A microbiology student notices that a culture broth tube was very turbid at the bottom of the tube but clear at the top of the tube. It can be concluded that the: - Organism should be grown in an anaerobic chamber - Organisms are aerobes - Organism can tolerate oxygen - Broth is sterile - Organism cannot produce superoxide dismutase and/or catalase 13. A characteristic of agar is: - It solidifies at 42 degrees C - A common source of nitrogen in liquid media - A polymer extracted from algae that has no nutritional value - It is solid at room temperature 14. Advantage of an agar slant preparation is: - Microbes can slide on agar easily - Less agar powder is needed - More surface area for growth of microbes 15. Which of the following instruments or equipment is used for sterilizing the media after it has been prepared? - Laminar air flow chamber - Inoculating needle - Incubator - Autoclave 16. A culture medium consisting of agar, human/sheep blood, and beef heart is a: - Complex media - Selective media - Reducing media - Chemically defined media - Differential media 17. What is the application of selective medium? - It allows growth of all the microbes inoculated to it - It allows the growth of one species of microorganisms while suppressing the growth of others - It does not allow the growth of any kind of microbes
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