how did we test if p and e DNA replicated the same
Added by Timothy C.
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This could involve using a model organism or a controlled in vitro system where both DNAs can be studied. Show more…
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Suppose that the DNA of cells (growing in a cell culture) in a eukaryotic species was labeled for a short period of time by the addition of $^{3} \mathrm{H}$ -thymidine to the medium. Next assume that the label was removed and the cells were resuspended in nonradioactive medium. After a short period of growth in nonradioactive medium, the DNA was extracted from these cells, diluted, gently layered on filters, and autoradiographed. If autoradiographs of the type were observed, what would this indicate about the nature of DNA replication in these cells? Why?
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