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How do you determine the enzyme activity conversion factor x/abs max in μmol? 

          How do you determine the enzyme activity conversion factor x/abs max in μmol?

Added by Soledad R.

Biology for AP Courses
Biology for AP Courses
Julianne Zedalis, John Eggebrecht
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How do you determine the enzyme activity conversion factor x/abs max in μmol?
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00:01 Hello students for the given question effect of temperature on enzyme activity enzyme activity was measured using a spectrophotometer the experiment was performed using the enzyme catalase which catalyzes the breakdown of hydrogen peroxide into water plus oxygen the amount of oxygen produced is directly proportion to enzyme activity the spectrophotometer measures the absorption of light at a specific wavelength which can be used to determine the concentration of substance in solution in this experiment a solution containing catalase was mixed with hydrogen peroxide and the reaction was allowed to proceed at different temperatures ranging from 0 to 80 degrees celsius at each temperature the…
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