How does anaphase I of meiosis differ from anaphase of mitosis? Sister chromatids line up at the metaphase plate in meiosis, whereas homologous chromosomes line up at the center in mitosis. Sister chromatids separate in meiosis, whereas homologous chromosomes separate in mitosis. Homologous chromosomes line up at the metaphase plate in meiosis, whereas sister chromatids line up at the center in mitosis. Homologous chromosomes separate in meiosis, whereas sister chromatids separate in mitosis.