§ How many enzymes above have “sticky ends” and why is that usually advantageous? (1)
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" Sticky ends are typically produced by restriction enzymes that cut DNA in a staggered manner, leaving overhanging sequences. Common examples include EcoRI, HindIII, and BamHI. Show more…
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Assertion: In rDNA technology, the restriction enzymes, those produce sticky ends are commonly used. Reason: Sticky ends facilitates the action of enzyme DNA ligase.
The text stated that molecular biologists have developed elegant techniques that can convert any type of DNA end into any other type of DNA end. In this problem, consider genomic DNA that is broken by mechanical shearing into random pieces. Some of the ends of these pieces are blunt, some have $5^{\prime}$ -overhangs, and others have $3^{\prime}$ -overhangs. a. Must the two ends of any one genomic DNA fragment be of the same type? b. Explain why the ends with $5^{\prime}$ or $3^{\prime}$ overhangs are not sticky. c. Researchers can convert ends with overhangs into blunt ends using either DNA polymerase (plus the four dNTPs), or nuclease S1, which degrades single-stranded regions of DNA but not doublestranded regions. Which kinds of ends with overhangs $\left(5^{\prime} \text { or } 3^{\prime}\right)$ could be converted into blunt ends using DNA polymerase? With S1 nuclease?
When a typical restriction enzyme cuts a DNA molecule, the cuts are staggered so that the DNA fragments have single-stranded ends. This is important in recombinant DNA work because the fragments will bond to other fragments with complementary single-stranded ends. The single-stranded ends serve as starting points for DNA replication. It allows cells to recognize fragments produced by the enzyme. Only single-stranded DNA segments can code for proteins.
Adi S.
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