00:02
Understanding this concept instead of actually recording data because i do not have a data for you to record.
00:10
So first of all, the idea asks you to measure the zone of inhibition, and you want to record a zone of inhibition's diameter.
00:18
And what is the point of doing this? so the idea is that you would actually put antibiotics on the piece of paper disk.
00:32
So the paper disk was being soaked with antibiotics.
00:36
And then the antibiotics soaked paper will be put in a dish.
00:44
And that you have a bacterial law.
00:48
But because you have the antibiotics sitting on that paper, it is inhibiting the growth of the bacteria around it.
00:56
So you'll actually see this ring here.
00:59
We call this zone of inhibition.
01:01
So the idea of inhibition means that inhibit the growth of the bacteria around it.
01:10
So the rest of other will be covered by bacteria.
01:15
So the idea is that if the bacteria resist or it's a resistance strain, let's just call this resistant strain.
01:35
And there's no inhibition or very small zone of inhibition because the antibiotics is not able to.
01:50
To kill the bacteria.
01:52
And then you have intermediate, which means that it has some kind of resistance, but it still will be able to kill.
02:00
So this is medium size of diameter.
02:06
When you have a susceptible strain, and this means that there will be a large zone inhibition, because the paper disk has the antibiotics that will be very effectively killing the bacteria around it.
02:25
So as you can see, measuring the bacteria the size of the zone of inhibition tell us that whether the bacteria belong to the antibiotic resistant strain, intermediate, or susceptible.
02:37
Now each antibiotics will have a table that tell you, basically a guideline to tell you what is the minimal diameter that is needed to claim a strain a strain or strain is resistant or susceptible.
02:59
So this is the concept of the first question.
03:05
And it tells you how to measure, for example, you will have, for example, you disk has a diameter of six and the clear has three widths beyond the disk...