You have a TE buffer that consists of 10 mM of Tris-Cl, pH of 7.6, and 1 mM of EDTA, pH 8.0. You need to prepare 1000 mL of TE BUFFER, and there are three stock solutions: 50 mM Tris-Cl and 0.5 M EDTA. How will you prepare the 1000 mL of TE? The answers that need to be filled in are below:
- You need to add ____ mL of Tris-Cl Stock.
- You need to add ____ mL of EDTA Stock.
- You need to add ____ mL of H2O.