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7. Set up semilog graph paper with distance migrated as the x (arithmetic) axis and log of base-pair length as the y (logarithmic) axis. Then, plot distance migrated versus base-pair length for each standard size marker DNA. 8. Connect data points with each other. 9. Locate on x axis the distance migrated by the first EcoRI fragment. Using a ruler, draw a vertical line from this point to its intersection with the standard curve. 10. Now extend a horizontal line from this point to the y axis. This gives the base-pair size of this EcoRI fragment. 11. Repeat Steps 9 and 10 for each HindIII and BamHI fragment. 12. Enter the base-pair size of EcoRI, HindIII, and BamHI fragments. Table X. Summary of the DNA fragments characterized by agarose gel electrophoresis. Size Marker EcoRI BamHI Hind III Distance Size Distance Size Distance Size Distance Size

          7. Set up semilog graph paper with distance migrated as the x (arithmetic) axis and log of
base-pair length as the y (logarithmic) axis. Then, plot distance migrated versus base-pair
length for each standard size marker DNA.
8. Connect data points with each other.
9. Locate on x axis the distance migrated by the first EcoRI fragment. Using a ruler, draw
a vertical line from this point to its intersection with the standard curve.
10. Now extend a horizontal line from this point to the y axis. This gives the base-pair
size of this EcoRI fragment.
11. Repeat Steps 9 and 10 for each HindIII and BamHI fragment.
12. Enter the base-pair size of EcoRI, HindIII, and BamHI fragments.
Table X. Summary of the DNA fragments characterized by agarose gel electrophoresis.
Size Marker
EcoRI
BamHI
Hind III
Distance Size
Distance Size
Distance Size
Distance Size

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7. Set up semilog graph paper with distance migrated as the x (arithmetic) axis and log of
base-pair length as the y (logarithmic) axis. Then, plot distance migrated versus base-pair
length for each standard size marker DNA.
8. Connect data points with each other.
9. Locate on x axis the distance migrated by the first EcoRI fragment. Using a ruler, draw
a vertical line from this point to its intersection with the standard curve.
10. Now extend a horizontal line from this point to the y axis. This gives the base-pair
size of this EcoRI fragment.
11. Repeat Steps 9 and 10 for each HindIII and BamHI fragment.
12. Enter the base-pair size of EcoRI, HindIII, and BamHI fragments.
Table X. Summary of the DNA fragments characterized by agarose gel electrophoresis.
Size Marker
EcoRI
BamHI
Hind III
Distance Size
Distance Size
Distance Size
Distance Size

Added by George R.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Jenny Wu

10/16/2023

Step 1

Step 1: First, measure the distance migrated for each standard size marker DNA (EcoRI, BamHI, HindIII) on the agarose gel. Show more…

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I need help completing this table. Base-pair length as the y logarithmic axis. Then plot distance migrated per base-pair length for each standard size marker DNA. 8. Connect data points with each other. A vertical line from this point to its intersection with the standard curve. 10. Now extend a horizontal line from this point to the y axis. This gives the base-pair size of this EcoRI fragment. 11. Repeat Steps 9 and 10 for each HindIII and BamHI fragment. 12. Enter the base-pair size of EcoRI, HindIII, and BamHI fragments. Table X. Summary of the DNA fragments characterized by agarose gel electrophoresis Size Marker EcoRI BamHI HindIII Distance Size Distance Size Distance Size Distance Size

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Transcript

00:01 So you have a standard curve that generated between the x -axis, which is the dna size in base pair, and the y -axis is going to be the distance traveled from the well in millimeter.

00:18 So you can see that this is a curve that you have a correlation between the size of dna and how far it travels.

00:25 You have an inverted correlation because it looks like when dna size goes up, the distance traveled, it travels, gets smaller.

00:36 So as you can see that we can use this standard curve to estimate the dna fragment by looking at the distance it traveled from the well.

00:47 So let's say band a, you have four bands on a gel and you want to use the distance they migrate indicate the table below to determine the size of the band by the curve.

01:03 The first one is about 25 millimeter.

01:07 So i'm trying to copy the figure as well as i could.

01:13 So you can see that when you have 25, it's somewhere here.

01:17 You have 30 in there and in between 20 and 40, 25 is again in between 20 and 30.

01:25 So if you look at the 25, it hits somewhere here.

01:35 So this tells us that the size of the band is correlated with something close to 700 base pair, which is in between 6 and 800 base pair.

01:53 The second band is about 30 millimeter.

01:59 So let's say 30 is slightly higher position somewhere here.

02:12 Actually, i'm not exactly straight.

02:19 It should fall right into about 600 base pair.

02:26 So when you have 30 millimeter travel distance, this correlate with about 600 base pair in dna size.

02:36 So you can see you go from the y axis and then you hit the standard curve.

02:40 It will tell you the x.

02:42 X will be the size of the dna.

02:45 The third one, 65...

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