00:01
We have three parts regarding the hplc, that is the high -performance liquid chromatography.
00:08
The first question here is, what is the primary function of hplc detector regardless of the type? so the hplc detectors, their main function is to detect the components that leave from the chromatographic column.
00:32
Right so this detector it converts this illusion of the components as electrical signals and stores the data right so basically they are mainly used to detect quantify and purify various analyze which are present in the sample right now the next question is what factors would you consider in choosing a hplc detector so the choice of hplc detectors it mainly, there are two types of detectors.
01:08
So there are specific detectors and then there are bulk property detectors.
01:18
So the specific detectors, they include a mass spectroscopy detector which works based on the fragmentation of molecules by electrical fields and separation of molecules based on mass to charge ratio.
01:32
And there are uv detectors.
01:33
So there are uv detectors, mass spectroscopic detectors, this photo diode array detectors and there are fluorescent detectors.
01:56
Right? so this is the answer for the third question.
02:05
All right.
02:06
That is the different types of detectors...