If a particular experiment were known to have a transformation efficiency of 3 x 10^3 bacteria/µg of DNA, how many transformant colonies would be expected to grow on the LB/amp/ara plate? You can assume that the concentration of DNA and fraction of cells spread on the LB agar are the same as that of the pGLO laboratory.
Added by Rebecca U.
Step 1
We know the transformation efficiency is 3 x 10^3 bacteria/µg of DNA. Show more…
Show all steps
Your feedback will help us improve your experience
Supreeta N and 101 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
After transformation, 65 colonies were present on the plate. What is the transformation efficiency of the competent cells if 120 ng of DNA was used for transformation of 100 µL of competent cells? LB medium was added to 1 mL total volume and 200 µL were plated after 1 hour growth at 37 degrees Celsius.
Adi S.
You transform some E. coli with 20 ng pAmp DNA. You get an average of 59 colonies when you plate 100 µl of the original 1 ml culture on media containing ampicillin. You calculate that the total cells in the original 1 ml was 2.12 x 10^9. What percentage of cells have been transformed? What is the transformation efficiency?
Sri K.
After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent cells if 100 ng of DNA was used for the transformation of 100 μl competent cells? LB medium was added to 1 ml total volume and 200 μl were plated after 1-hour growth at 37°C. What was your transformation efficiency for control DNA pTUD at a concentration of 10 ng/μl? What was the transformation efficiency for experimental ligation (you will need to calculate the approximate concentration from the steps of the cloning procedure you performed)?
Derrick D.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD