If a sample contains 400 µM of PNP,how can you dilute this sample to 2 µM using a serial dilution? The final volume of each dilution in the series must be 5 mL.For each dilution in the series; state the volumes of PNP and buffer required (in mL to one decimal place) and the concentration of PNP after each dilution (in µM to the nearest whole number).
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