If instead you were to make a contiguous coding sequence of lipase joined to a 3’ His tag, which part of the lipase coding sequence should not be included in the PCR product? Question 6Answer a. polyA tail b. start codon c. 5' UTR d. stop codon
Added by Kenneth R.
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A typical coding sequence includes the start codon, the coding region, and the stop codon. Additionally, there are untranslated regions (UTRs) at both the 5' and 3' ends. Show more…
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1. "A" (the 5' UTR) includes the A. promoter B. 5' cap C. start codon D. Shine-Dalgarno sequence E. None of the above 2. "B" (the coding region) includes A. introns and exons B. 5' and 3' splice sites C. a stop codon D. a binding site for Sigma Factor E. All of the above 3. "C" (the 3' UTR) includes A. a palindrome B. a stop codon C. a binding site for RNA polymerase D. a TRANS element E. None of the above
Keemin L.
Which would not be found on a mature mRNA molecule? a. protein-coding region b. start and stop codons c. promoter d. 3' untranslated region e. 5' untranslated region
Rabeya Z.
The following segment of DNA encodes a polypeptide containing six amino acids. DNA triplets encoding the translation initiation (or start) codon and a stop codon are included in the sequence. 5' - ...CCCAGCCTAGCCTTTGCAAGAGGCCATATCGAC... -3' 3' - ...GGGTCGGATCGGAAACGTTCTCCGGTATAGCTG... -5' a. Label which of the DNA strands is the template strand and which is coding strand. b. What is the sequence and the polarity of the mRNA encoded by this 'gene'? c. The Genetic code table is provided at the end of the exam. Determine the amino acid sequence of the polypeptide (use three letter codes for the amino acids) and identify the N— and C—terminal ends of the polypeptide. d. Which of the 6 side chains in this polypeptide can form hydrogen bonds with polar molecules (like water)? Place a circle around these. e. A base-pair substitution mutation occurs (as shown by the bold nucleotides below). How does this change affect the polypeptide sequence? Show your work. 5' - ...CCCAGCCTAGCCTTTTCAAGAGGCCATATCGAC... -3' 3' - ...GGGTCGGATCGGAAAAGTTCTCCGGTATAGCTG... -5'
Jenny W.
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