If the circular DNA were nicked (had a single-stranded break) on one strand, what would be the effect on supercoiling?
Added by Kyle A.
Step 1
** Show more…
Show all steps
Your feedback will help us improve your experience
Madhur L and 55 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Consider a relaxed, covalently-closed circular DNA plasmid that has 1040 base pairs with Wr = 0. An intercalator is added, such that there is one intercalator per 104 base pairs. The effect of the intercalator is to cause the twist between the base pairs that flank it to be reduced to zero. What will be the resulting change in supercoiling for the intercalator-bound DNA?
Madhur L.
You have discovered an enzyme secreted by a particularly virulent bacterium that cleaves the C2'-C3' bond in the deoxyribose residues of duplex DNA. What is the effect of this enzyme on supercoiled DNA?
Sri K.
(a) What is the $L k$ of a 5,000 bp circular duplex DNA molecule with a nick in one strand? (b) What is the $L k$ of the molecule in (a) when the nick is sealed (relaxed)? (c) How would the $L k$ of the molecule in (b) be affected by the action of a single molecule of $E .$ coli topoisomerase I? (d) What is the $L k$ of the molecule in $(b)$ after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP? (e) What is the $L k$ of the molecule in (d) after four enzymatic turnovers by a single molecule of bacterial type I topoisomerase? (f) What is the $L k$ of the molecule in (d) after binding of one nucleosome?
Lainey R.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD
