If you wanted to deliver a gene that inserts into the genome, which gene therapy vector would you choose? O herpes simplex virus O liposome O lentivirus O adenovirus
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Fill in the Blank . _____________ is a common viral vector used in gene therapy for introducing a new gene into a specifically targeted cell type.
The adenovirus has been genetically engineered to act as a vector to bring genes into the cells for human gene therapy. Suggest a virus that could be used as a vector to carry genes into plants for plant gene therapy.
Luke H.
What is a feature peculiar to adeno-associated viral (AAV) vectors and not shared with retroviral vectors? Therapeutic gene integrates randomly into the patient's genome and could lead to cancer. Packaging the therapeutic DNA into virus-like particles requires "helper DNA" which expresses all the proteins required to make viral particles. Therapeutic gene is transcribed and translated in patient cells using the host transcription and translation machinery. Packaging cells do not make active viruses because helper DNA lacks packaging sequences and therapeutic DNA lacks essential viral genes. Gene therapy needs to be repeated periodically to provide a constant supply of the therapy protein.
Josee P.
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