In a SEQUENTIAL order, whit are the three steps in PCR? A. Anneal Primers, Extend DNA, Denature DNA B. Denature DNA, Extend DNA, Anneal Primers C. Denature DNA, Anneal Primers, Extend DNA D. Extend DNA, Anneal Primers, Denature DNA
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The steps involved in a single PCR cycle, in the correct order, are A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA. B) heat to denature DNA strands, cool to allow primer to anneal, heat slightly to allow DNA polymerase to copy DNA. C) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA. D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
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A. What are the three main steps of a PCR cycle, and what is happening at each step? B. How do the PCR primers differ from the primers used in DNA replication in a cell? C. Why is Taq DNA polymerase used in PCR?
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Each PCR cycle involves three distinct steps. Name each of the following steps of a PCR cycle: a. Unwinding of DNA b. Primer attachment to the template DNA c. Taq polymerase attachment and DNA synthesis
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