In an assay for a dehydrogenase you observe an increase in the absorbance at 340 nm of 0.5 due to the production of NADH. Using the extinction coefficient that you determined in problem 4, calculate the change in concentration of NADH (in mM). For a 1 cm pathlength cuvette this translates to an extinction coefficient of 9.8765 mM-1 cm-1
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Step 1: Calculate the change in concentration of NADH using the formula: Change in concentration of NADH (in mM) = (Absorbance at 340 nm) / (Extinction coefficient x Path length) Show more…
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