00:01
So basically here, as the first time, we have the time.
00:09
Basically on the time we have 10 minutes, 20, 30, 40, 50, 50, and 60.
00:15
So here, as we can see that we have the gene which got incorporated.
00:19
We have e, a plus e, a plus b plus c, a plus b plus t plus e.
00:26
We have a plus b plus c plus c plus e.
00:31
And we have a plus b plus c plus d plus e plus f.
00:36
As the first gene to enter is e, it must be the last gene present on bacterial chromosome, and the last gene is considered as a selection marker so as to prevent the recipient cells from growing on the plates along with the x conjugants.
00:52
Now, seeing the order of genes, the sequence can be interpreted.
00:55
If you let the ret to be the site of nic for transformation, the direction determines the order of gene transport as it is bacterial chromosome...