Question

In Gram Stain, crystal violet functions as the primary stain. Once this is added, Gram positive cells look purple while Gram negative cells are colorless. Next, the mordant iodine is added and all the cells look the same as in the previous step. The next step is the addition of ethanol, which functions as the decolorizer. It is at this point that Gram positive cells look purple while Gram negative cells look colorless. Addition of the secondary dye safranin will make Gram positive cells look purple and Gram negative cells look pink. However, if ethanol is added for too long, Gram negative cells look purple while Gram positive cells look pink.

          In Gram Stain, crystal violet functions as the primary stain. Once this is added, Gram positive cells look purple while Gram negative cells are colorless. Next, the mordant iodine is added and all the cells look the same as in the previous step. The next step is the addition of ethanol, which functions as the decolorizer. It is at this point that Gram positive cells look purple while Gram negative cells look colorless. Addition of the secondary dye safranin will make Gram positive cells look purple and Gram negative cells look pink. However, if ethanol is added for too long, Gram negative cells look purple while Gram positive cells look pink.

Added by Daniel G.

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