00:02
So you know that you have a version of a beta -autonomous experiment that you tend to test one gene, one enzyme hypothesis, but you use the pathway of synthesis of amino acid q from x.
00:18
So we know that you have this pathway, x is always present in cell and it converts into y through e1 enzyme and then y converts to z by enzyme 2 and z converts to p by e3 enzyme and p converts to d by enzyme e4 and d converts to q by e5.
00:45
And of course, q is required for cells to grow.
00:48
So you identify an auxotroph that doesn't grow in the normal medium, but it does grow in the medium supplement with q.
00:55
So this tells us that obviously there is a defect in one or more genes.
00:59
But if you also provide this medium with y, the auxotroph can also grow.
01:06
So does it support the one gene and one enzyme hypothesis? obviously the answer is yes.
01:13
So this means that, first of all, this is an auxotroph that it cannot grow on the regular medium because it's missing q.
01:21
The reason that we miss q is one of the enzymes in this pathway stopped working...