In the DNA extraction, you used papain in order to digest away carbohydrates surrounding the genetic material. keep the DNA from precipitating out of the solution. break up proteins associated with the DNA. separate lipids from the DNA. break apart the plasma membrane and the nuclear envelope.
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Put the steps of DNA extraction in order. Washing nucleic acids Mechanical and physical breakdown of tissue Separation of nucleic acids from lipids and proteins Re-dissolving pellet for storage Precipitation and aggregation of nucleic acids out of solution Match the substances with their function in DNA extraction Disturb the phospholipid bilayer to lyse cells Separate nucleic acids from lipids and proteins Precipitate nucleic acids and complete separation from other hydrophilic solutes Aggregate or clump nucleic acids Wash nucleic acid pellet to remove salts and remaining ions 1. Isopropanol 2. Sodium acetate 3. CTAB 4. Chloroform 5. Ethanol
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What is the function of the salt in the DNA extraction lab? Choose only one group of answer choices: - Help dissolve the phospholipid bilayers of the cell membrane and organelles - Isolate the DNA - Denature the DNA - Help break up protein chains that bind around the nucleic acids
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When extracting DNA, there were several important steps that you took in order to result in a purified DNA product. Which of the following were among these steps? a. To stain the DNA blue so that you were able to visualize it better. b. To break open cell membranes and nuclei so that the DNA would be released into solution. c. To add a protease to degrade proteins which might bind to DNA. d. To add a DNA endonuclease that would cut the DNA into manageable-sized pieces. e. both a, b, and c. f. both b and c. g. both b, c, and d. h. both a, b, c, and d.
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