In the enzyme lab, we added liver to the hydrogen peroxide. Hydrogen peroxide broke down to produce oxygen gas and water with the use of catalase. Indicate the substrate(s), enzyme(s) and product(s) in this experiment. Substrate(s): Product(s): Enzymes(s): Would catalase enzyme work on lactose? Why or why not?
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You are studying enzymes in biology class. Hydrogen peroxide (H2O2) is produced as a byproduct of respiration. Hydrogen peroxide is harmful to cells and must be continually broken down. Catalase is an enzyme found in living cells that speeds up the breakdown of hydrogen peroxide into water and oxygen (2 H2O2 → 2 H2O + O2). You are working with your lab group to investigate what factors influence the rate of the peroxide reaction. Your lab group is provided with liver samples as a source of catalase, as well as 3% hydrogen peroxide. You decide to investigate if adding more enzyme will cause the reaction to proceed faster. You set out to prove the hypothesis: if more catalase is provided, then the rate of the peroxide reaction will be faster. What would be a good choice for your independent variable? a) 3, 5, and 10 g samples of liver b) measuring the amount of oxygen produced c) 1%, 3%, and 10% concentrations of hydrogen peroxide d) 10 mL, 20 mL, and 30 mL volumes of hydrogen peroxide.
Jorge V.
Catalase 2 H2O2 --------> 2 H2O + O2 Hydrogen Water Oxygen Peroxide In this chemical reaction, what is the substrate? 2. What are the products? 3. What is the enzyme? 4. Which of the above molecules would fit into the active site of catalase?
Shaiju T.
Questions $90-93$ Students do an experiment to explore the effects of $\mathrm{pH}$ on enzyme activity. They use the enzyme catalase, which is found in all aerobic cells. The function of catalase is to break down the harmful waste product of cellular respiration, hydrogen peroxide $\left(\mathrm{H}_{2} \mathrm{O}_{2}\right),$ into oxygen gas and water. The reaction looks like this: $2 \mathrm{H}_{2} \mathrm{O}_{2} \rightarrow 2 \mathrm{H}_{2} \mathrm{O}+\mathrm{O}_{2}$ The students set up 10 tests tubes, each containing 5 $\mathrm{mL}$ of peroxide. They adjust the $\mathrm{pH}$ of each test tube as shown below. To test tubes $\# 1-$ 5 they add 3 $\mathrm{mL}$ of catalase. To test tubes $\# 6-10,$ they add 3 $\mathrm{mL}$ of distilled water. Immediately, some of the tubes begin to bubble. The students use a glowing splint to test for the presence of oxygen and to prove that the bubbles contain oxygen gas. Their data is in the chart below. TABLE CANNOT COPY Which organelle is most closely linked to hydrogen peroxide $\left(\mathrm{H}_{2} \mathrm{O}_{2}\right) ?$ (A) Nucleus (B) Ribosome (C) Nucleolus (D) Golgi body (E) Mitochondria
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