In the salt-out method, what role do salts play in the protein purification process? Precipitation of proteins Activation of proteins Denaturation of proteins Solubilization of proteins
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What is the function of the salt in the DNA extraction lab? Choose only one group of answer choices: - Help dissolve the phospholipid bilayers of the cell membrane and organelles - Isolate the DNA - Denature the DNA - Help break up protein chains that bind around the nucleic acids
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Explain how you would use salting out to prepare a more concentrated solution of a protein.
In the process of salting-in, protein solubility in a dilute salt solution is increased by adding more salt. Because the protein solubility depends on the total ion concentration as well as the ion charge, salts containing doubly charged ions are often more effective than those containing singly charged ions. (a) How many grams of $\mathrm{MgCl}_{2}$ must dissolve to equal the ion concentration of 12.4 $\mathrm{g}$ of $\mathrm{NaCl} ?$ (b) How many grams of CaS must dissolve? (c) Which of the three salt solutions would dissolve the most protein?
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