Question

In vitro transcription reactions can be performed by mixing general transcription factors, RNA polymerase II, nucleotides, and a DNA template; however, genomic DNA does not work when used as the DNA template in these reactions. Why? My question is, what is the relation to the euchromatin?

Name: in vitro transcription reactions can be performed by mixing general transcription factors rna polymerase ii nucleotides and a dna template however genomic dna does not work when used as the 12635
Uploaded: 2023-08-10T13:03:36-08:00
Duration: 3 min 34 s
Channel: Madhur L
Description: in vitro transcription reactions can be performed by mixing general transcription factors rna polymerase ii nucleotides and a dna template however genomic dna does not work when used as the 12635

          In vitro transcription reactions can be performed by mixing general transcription factors, RNA polymerase II, nucleotides, and a DNA template; however, genomic DNA does not work when used as the DNA template in these reactions. Why? My question is, what is the relation to the euchromatin?

Added by Michael T.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Madhur L

08/10/2023

Step 1

These regions are characterized by a higher accessibility of DNA to transcription factors and RNA polymerase, which allows for efficient transcription of genes. Now, when we use genomic DNA as a template for in vitro transcription reactions, we are essentially Show more…

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In vitro transcription reactions can be performed by mixing general transcription factors, RNA polymerase II, nucleotides, and a DNA template; however, genomic DNA does not work when used as the DNA template in these reactions. Why? My question is, what is the relation to the euchromatin?