Indicate if each case will result in INCREASED or DECREASED signaling. Explain why. 1. G-protein Coupled Receptor mutation that causes a 1000-fold increase in receptor's affinity for ligand. 2. Mutations that result in disulfide bond formation between the Gα and Gβ subunits of the heterotrimeric G-protein. 3. A 10-fold increase in the half-life of cAMP 4. Mutations in the kinase domain of Tyrosine Kinase Receptors that abolish its activity. 5. Mutation in βARR that decreases its affinity for the phosphorylated receptor cytoplasmic tail.
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This is because the receptor will be able to bind to the ligand more effectively, leading to a stronger signal transduction. Show more…
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