Indicate whether you would expect growth of the obligate...

Indicate whether you would expect growth of the obligate anaerobe to occur under the following circumstances and a brief explanation why: An obligate anaerobe is inoculated into a tube of fluid thioglycolate medium and placed in an incubator. A strictly fermentative aerotolerant anaerobe is co-inoculated into nutrient broth with an obligate anaerobe and placed in an incubator.

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00:01 All right, suppose we have a tube of thioglycolate media, and we basically are trying to see if which in which conditions would these two bacterial bacteria would grow.

00:11 Sorry.

00:13 Essentially, we inoculate the thioglycolate broth, and then we look at the growth after some time it's been in the incubator.

00:22 Now, i would like to stress that, theoretically speaking, if you have an anaerobic bacteria, you're growing it in thioglycolate broth.

00:28 You're not really growing it in aerobic conditions for very long, because eventually, oxygen will penetrate to all regions of the thioglycolate broth if it's left out long enough, right? but, assuming that that doesn't happen, the first scenario is in which we have an obligate anaerobe that's inoculated into this thioglycolate media.

00:50 Oh, you know what i should also do? let me describe something real quick.

00:54 Thioglycolate, the way thioglycolate works is that there is reducing agents in the medium, the thioglycolate really, such that there is more oxygen content at the top than there is at the bottom.

01:18 Meaning, at this interface, there's more oxygen present at this interface, and it decreases as we go lower, such that we'll have essentially an o2 -rich, otherwise known as an o2 -replete condition, and at the very bottom, we'll have an o2 -poor, or a depleted oxygen condition.

01:59 All right? so, that's essentially how thioglycolate works.

02:06 So, if we have an obligate anaerobe, which i'll label in blue, and it's inoculated into this thioglycolate medium, and it's placed in an incubator, i would expect it to grow here, okay? remember, this is an obligate anaerobe, and so it will cluster towards the bottom where there is less oxygen, if no oxygen, because it's an obligate anaerobe, so it cannot survive in oxygenated environments...

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