1. IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Using your knowledge of how the lac operon is regulated, predict whether these gene products (? -galactosidase, lactose Permease) will be produced (yes (+)/no (-)) in the presence or absence of the inducer IPTG in the following partial diploids +IPTG -IPTG ?-galactosidase Permease ?-galactosidase Permease (A) I+P-O+Z-Y+/ I-P+O^cZ+Y- (B) I^sP+O^cZ+Y-/ I+P+O+Z-Y+ (C) I+P+O+Z-Y+/ I-P+O+Z+Y- (D) I+P+O^cZ-Y+/ I^sP+O+Z+Y+ (E) I+P-O^cZ+Y+/ I-P+O+Z+Y+
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Step 1: In the presence of IPTG (+IPTG), the lac operon is induced, allowing for the production of β-galactosidase and lactose permease. Show more…
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IPTG is a molecule with a structure very similar to lactose. IPTG can be transported into cells by galactoside permease and can bind to the lac repressor protein. However, unlike lactose, IPTG is not broken down by $\beta$ -galactosidase. Predict what would occur to lac operon regulation if IPTG were added to $E$. coli growth medium containing no glucose or lactose.
IPTG is a molecule with a structure very similar to lactose. IPTG can be transported into cells by galactoside permease and can bind to the lac repressor protein. However, unlike lactose, IPTG is not broken down by $\beta$ -galactosidase. Predict what would occur regarding lac operon regulation if IPTG were added to $E$. coli growth medium containing arabinose and no glucose or lactose.
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Study the depiction of the lac operon in Figure $11.2 .$ Normally, the genes are turned off when lactose is not present. Lactose activates the genes, which code for enzymes that enable the cell to use lactose. Predict how the following mutations would affect the function of the operon in the presence and absence of lactose: a. mutation of regulatory gene; repressor will not bind to lactose b. mutation of operator; repressor will not bind to operator c. mutation of regulatory gene; repressor will not bind to operator
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