Text: ISOLATION METHODS QUANTIFICATION BY COLONY COUNTING
Introduction
Laboratory Simulation
Using the count data and observational data you acquired, calculate the number of CFUs in the original sample.
Number of CFUs
CFUs
You discover that the plate you selected had only been inoculated with 0.1 mL of the dilution instead of 1 mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original sample.
Number of CFUs
CFUs
Lab Data
Phase 1 Isolated Colonies Present? Countable? Plate Dilution Label Growth Appearance on Plate Too numerous to count 1,000,000 Heavy Yes (even) Too numerous to count 1,000,000 Heavy Yes; Many Countable 100,000 Intermediate Yes (lex) Too numerous to count 10,000,000 Scant Save Image Image
Phase 2 Plate Selected For Counting Volume Diluted Sample (mL) Sample Dilution
Plate
1,000,000
Colony Count