It is very important that you are familiar with the presentation of bacterial growth data. Below is a set of data that you need to plot and to complete the following tasks: Use to the following conversion factor to determine the average number of cells present in your sample at every time point. It is very important that you are familiar with the presentation of bacterial growth data. Below is a set of data that you need to plot and to complete the following tasks: at every time point. OD600nm 0.1=1x10cfuml Time (min) OD600nm Set2 0.044 0.048 0.049 0.047 0.081 0.19 0.40 0.78 0.96 0.98 0.95 1.01 1.12 1.01 0.98 1.05 1.10 1.05 Set1 0.054 0.052 0.047 0.049 0.086 0.22 0.36 0.81 0.98 0.94 0.90 0.99 0.86 0.94 0.90 0.98 0.92 0.92 Set3 0.05 0.048 0.048 0.051 0.095 0.20 0.37 0.83 0.99 1.04 1.20 0.98 1.05 1.10 1.05 0.99 1.03 1.22 0 30 60 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 2. Determine the number of cells present in your sample at t=200min
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Step 1: Convert the OD600nm values to the number of cells using the conversion factor provided. Show more…
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(A) From the provided data below at Microbes/Microorganisms 1 and 2, correlate the microbial/bacterial counts (number of cells) vs. the time to the Microbial Standard Growth Curve. (B) Plot Microbes/Microorganisms 1 and 2. Interpret the plotted data through analyzing and comparing each microbe's (number of cells). NOTE: ***MICROBIAL/BACTERIAL COUNTS (NUMBER OF CELLS) IS EQUIVALENT TO NUMBER OF BACTERIA*** DATA: MICROORGANISMS or MICROBES 1 Duration (time in minutes) Microbial/bacterial counts (number of cells) 0 5 x 10^4 30 1 x 10^5 60 2 x 10^5 90 4 x 10^5 120 8 x 10^5 150 1.6 x 10^6 180 3.2 x 10^6 210 6.4 x 10^6 240 1.28 x 10^7 MICROORGANISMS/MICROBES 2 Duration (time in minutes) Microbial/bacterial counts (number of cells) 0 4 x 10^3 20 1 x 10^4 40 2 x 10^4 60 4 x 10^4 80 8 x 10^4 100 1.6 x 10^5 120 3.2 x 10^5 140 6.4 x 10^5 160 1.28 x 10^6 180 2.56 x 10^6 PLOT FORMAT: X-AXIS (DURATION-TIME IN MINUTES): 30 (leftmost portion of the x-axis), 60, 90, 120, 150, 180, 210, & 240 (rightmost portion of the x-axis) Y-AXIS (Microbial/bacterial counts-number of cells): 10^4 (lowest part of the y-axis), 10^5, 10^6, 10^7, & 10^8 (uppermost part of the y-axis)
Shaiju T.
BIOL 401 General Microbiology Bacterial population that increases over time What is the generation time in hours? What is the constant (K) in this situation? What is the mean growth rate? A student counted an average of 12 cells per square inch for a bacterial culture using a Petroff-Hausser counting chamber when the optical density at 600 nm is 0.5 to 0.8 after eight hours. How many cells per mL should be present in the culture now? What is the mean growth rate constant (K) for this culture? You performed spectrophotometric measurements to determine the growth rate of a bacterial cell culture and obtained the data shown below. Please plot the absorbance versus time on a logarithmic (base 10) scale to show the growth curve as a straight line. Find the slope of the line and calculate the generation time from the slope. You will first need to calculate the time elapsed (duration) for some of the data points shown in the table below: Duration (min) OD (optical density) 3.59 AM 0.208 5.59 0.227 8.59 0.245 10.59 0.262 12.59 PM 0.280 14.59 0.302 16.59 0.357 18.59 0.384
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Below is the data collected from a closed-system growth curve of V. natriegens at varying salt concentrations (0 mM, 50 mM, 250 mM, and 1000 mM NaCl). Using the OD 600 nm values and the McFarland standard curve, determine the number of bacterial cells/mL at each time point. Once you've calculated the number of bacterial cells/mL, plot the growth curves of V. natriegens in BHI with 0 mM, 50 mM, 250 mM, 1000 mM salt (# bacterial cells/mL versus time). These graphs MUST be plotted using SEMI-LOG graph paper. Growth curves can either be plotted on one graph or 4 separate graphs. All graphs should be properly labeled. Y-axis = # bacterial cells/mL and the X-axis = time (min). Semi-log graph paper is available on Canvas. You may also choose to use Excel.
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