00:02
Let us consider methyl red test and oaks -pasca test that is used to differentiate.
00:16
Mrvp test is used to differentiate faculty to anaerobic, entric bacteria, especially e.
00:32
Coli, and among this centric bacteria, we try to differentiate between e.
00:42
Coli and entrobacter erosins.
00:49
Entrobacter erosines and e.
00:55
Coli.
00:55
Esceracea coli.
00:57
Let us say this one is for mr and this one is for vp.
01:02
Mr stands for methyl dead test.
01:03
Vp stands for vox -basker test.
01:07
Entrobacter aerogenes is negative for mr test, positive for vp test.
01:13
E -coli is positive for mr test, negative for vp test.
01:21
Now let us consider the fermentation here.
01:25
Glucose second question.
01:30
Here, glucose is used by entrobacter erosines and it forms acetoyene which is detected by vp test, oaks -pastker test, hence it gives positive result for vp test.
01:54
Whereas, eceracia uses glucose and forms acid.
02:00
Acid detection is done using methylasekate.
02:02
Methyl red dye, hence methyl red is positive for e.
02:11
Coli.
02:14
Now let us consider the second person here.
02:18
The fermentation of glucose.
02:24
How glucose is fermented by entrobacter aerosines and by e.
02:39
Coli.
02:40
Let us consider this.
02:45
Glucose, it is hydrolyzed to two pyrovate molecules these two pyruite molecules are used by endrobacter erosins to synthesize acetyl co -a, 2 -3, butane -dol, ethanol, lactic acid, formic acid, which are further converted to carbon dioxide, hydrogen, and ph is 6...