Question

LAB -4 BACTERIAL GROWTH 1) Sketch a growth curve for batch growth of E.coli with X axis showing growth time and Y axis showing number of cells. Label Phases of growth. 2) How do you think the above curve will change if your experiment involved observing bacterial growth in a CFSTR with a 0.2 micron filter at both the inlet and outlet? The flow coming in has a constant concentration of substrate S. 3) What is the average doubling time of E.coli? What factors can cause changes in this value? 4) Graphically represent how the concentration of S and X vary over time in a batch reactor (case in question 1) and in the CFSTR (case in question 2). State reasons to support your answer. 5) During an experiment a biologist grows bacteria to study the effects of microbial adhesion on different surfaces. At time t =0 hours he inoculates 100 bacteria into a favorable growth medium. Seven hours later he finds 1100 bacteria. (Assume exponential growth; Use first order rate model). a. Determine the growth constant 'k' for bacterial growth. b. How long does it take for the bacterial numbers to increase to 20 times its initial value? 6) When determining the trend of a growth curve by turbidity methods, how will turbidity change at the onset and progress of the death phase? Why?

          LAB -4 BACTERIAL GROWTH

1) Sketch a growth curve for batch growth of E.coli with X axis showing growth time and Y axis showing number of cells. Label Phases of growth.
2) How do you think the above curve will change if your experiment involved observing bacterial growth in a CFSTR with a 0.2 micron filter at both the inlet and outlet? The flow coming in has a constant concentration of substrate S.
3) What is the average doubling time of E.coli? What factors can cause changes in this value?
4) Graphically represent how the concentration of S and X vary over time in a batch reactor (case in question 1) and in the CFSTR (case in question 2). State reasons to support your answer.
5) During an experiment a biologist grows bacteria to study the effects of microbial adhesion on different surfaces. At time t =0 hours he inoculates 100 bacteria into a favorable growth medium. Seven hours later he finds 1100 bacteria.
(Assume exponential growth; Use first order rate model).
a. Determine the growth constant 'k' for bacterial growth.
b. How long does it take for the bacterial numbers to increase to 20 times its initial value?
6) When determining the trend of a growth curve by turbidity methods, how will turbidity change at the onset and progress of the death phase? Why?

LAB -4 BACTERIAL GROWTH

1) Sketch a growth curve for batch growth of E.coli with X axis showing growth time and Y axis showing number of cells. Label Phases of growth.
2) How do you think the above curve will change if your experiment involved observing bacterial growth in a CFSTR with a 0.2 micron filter at both the inlet and outlet? The flow coming in has a constant concentration of substrate S.
3) What is the average doubling time of E.coli? What factors can cause changes in this value?
4) Graphically represent how the concentration of S and X vary over time in a batch reactor (case in question 1) and in the CFSTR (case in question 2). State reasons to support your answer.
5) During an experiment a biologist grows bacteria to study the effects of microbial adhesion on different surfaces. At time t =0 hours he inoculates 100 bacteria into a favorable growth medium. Seven hours later he finds 1100 bacteria.
(Assume exponential growth; Use first order rate model).
a. Determine the growth constant 'k' for bacterial growth.
b. How long does it take for the bacterial numbers to increase to 20 times its initial value?
6) When determining the trend of a growth curve by turbidity methods, how will turbidity change at the onset and progress of the death phase? Why?

Added by Nicol-S S.

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