Question

Lab Report: Isolation of Plasmid DNA from Bacteria 4. If your plasmid is 5,000 bp and a restriction enzyme cuts it once, how many bands would you expect to see on agarose gel electrophoresis? Explain your answer. I would expect to see one band on the agarose gel electrophoresis. Since a 5,000 bp plasmid is cut once, it will result in a single band. 5. If you cloned a 300 bp fragment of DNA into the 5,000 bp plasmid, how many bands and what size bands would you expect to see if you cut this plasmid with the restriction enzyme? Explain WHY? 6. If each of two different restriction enzymes cuts a plasmid once, how many bands would you expect to see on an agarose gel and WHY?

          Lab Report: Isolation of Plasmid DNA from Bacteria

4. If your plasmid is 5,000 bp and a restriction enzyme cuts it once, how many bands would you expect to see on agarose gel electrophoresis? Explain your answer. I would expect to see one band on the agarose gel electrophoresis. Since a 5,000 bp plasmid is cut once, it will result in a single band.

5. If you cloned a 300 bp fragment of DNA into the 5,000 bp plasmid, how many bands and what size bands would you expect to see if you cut this plasmid with the restriction enzyme? Explain WHY?

6. If each of two different restriction enzymes cuts a plasmid once, how many bands would you expect to see on an agarose gel and WHY?

Added by Mariano R.

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