Label 1 automated sequencer reads output 2 dna polymerase 3...

Label: 1. Automated sequencer reads output 2. DNA polymerase 3. Synthesis of new DNA until incorporation of a ddNTP 4. Capillary electrophoresis of fragments and detection of fluorescent labels Details: - dATP - ddCTP - dGTP - ddGTP - ddTTP - dTTP - ddATP - dCTP - 3' ACGTTCAGT5 - 5' TGCAAGTCA 3' (a copied strand would read: 5' TGCAAGTCA 3') - Longer fragments - Single-stranded template - Shorter fragments

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Transcript

00:01 Hello everyone.

00:02 The main concept of this question is sangha sequencing.

00:07 Sanga sequence.

00:16 Let us explain briefly about the sanga sequencing.

00:21 Sanga sequencing is a method that is used to determine the nucleotide sequence.

00:27 Its main function is the determination of nucleotide sequence.

00:45 The sanga sequencing is also known.

00:48 As the chain terminating method.

00:51 The chain terminating method.

01:01 This question it is asked that we must sequentially arrange the steps that are involved in the sangha's sequencing.

01:11 There are four major steps which are used here.

01:15 Four steps.

01:19 Let us read those four steps quickly.

01:22 First in step one, the dna sequence of interest the dna sequence of interest is used as a template.

01:44 The dna sequence of interest is used as a template.

01:48 Here is where the addition of ddntps occur.

01:54 Here is where the addition of dd npp is occur.

02:00 Now you can ask me what is the dd ntps occur.

02:07 D d ntps is it stands for di -deoxy nucleotides di -deoxy nucleotides this ddntpi -ntpi it is used to terminate growing dna chains it is used to terminate growing dna chains this is the first step followed by the second step...

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